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Federation of European Neuroscience Societies actinomycin d (act-d)
Actinomycin D (Act D), supplied by Federation of European Neuroscience Societies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ApexBio actinomycin d actd
Actinomycin D Actd, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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FUJIFILM actinomycin d (cat.#018-21264)
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Actinomycin D (Cat.#018 21264), supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actinomycin+d+act+d/actinomycin+d+act+d/pmc09224560-80-0-6
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FUJIFILM Wako Pure Chemical Co actinomycin d actd
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Actinomycin D Actd, supplied by FUJIFILM Wako Pure Chemical Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Applichem inc transcription inhibitor actinomycin d
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Transcription Inhibitor Actinomycin D, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LC Laboratories actinomycin d act d
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Actinomycin D Act D, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CH Instruments actinomycin d act d
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Actinomycin D Act D, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/actinomycin+d+act+d/actinomycin+d+act+d/pm24414258-177-5-11
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Bio-Techne corporation actinomycin d
COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Actinomycin D, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Elevated Alpha 1(I) to Alpha 2(I) Collagen Ratio in Dermal Fibroblasts Possibly Contributes to Fibrosis in Systemic Sclerosis

doi: 10.3390/ijms23126811

Figure Lengend Snippet: COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.

Article Snippet: Actinomycin D (Cat.#018-21264) was purchased from Wako (Osaka, Japan).

Techniques: Transfection, Real-time Polymerase Chain Reaction, Incubation, Expressing

COL1A1 overexpression affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector were determined using real-time PCR. The mean value of the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control ( B ) Protein levels of type I collagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector are shown. The graph shows the mean relative type I collagen levels. The mean value in the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with virus-containing medium with a control or COL1A1 expression vector were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Elevated Alpha 1(I) to Alpha 2(I) Collagen Ratio in Dermal Fibroblasts Possibly Contributes to Fibrosis in Systemic Sclerosis

doi: 10.3390/ijms23126811

Figure Lengend Snippet: COL1A1 overexpression affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector were determined using real-time PCR. The mean value of the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control ( B ) Protein levels of type I collagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector are shown. The graph shows the mean relative type I collagen levels. The mean value in the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with virus-containing medium with a control or COL1A1 expression vector were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.

Article Snippet: Actinomycin D (Cat.#018-21264) was purchased from Wako (Osaka, Japan).

Techniques: Over Expression, Expressing, Plasmid Preparation, Real-time Polymerase Chain Reaction, Transfection, Incubation