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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Elevated Alpha 1(I) to Alpha 2(I) Collagen Ratio in Dermal Fibroblasts Possibly Contributes to Fibrosis in Systemic Sclerosis
doi: 10.3390/ijms23126811
Figure Lengend Snippet: COL1A1 silencing affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA were determined using real-time PCR. The mean values in the control siRNA treatment were set as 1 (n = 3 per group). * p < 0.05 versus the control. ( B ) Protein levels of type I collagen in control fibroblasts transfected with COL1A1 or COL1A2 siRNA are shown. The graph shows the mean relative type I collagen levels. The mean value in control siRNA was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with COL1A1 or control siRNA were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Article Snippet:
Techniques: Transfection, Real-time Polymerase Chain Reaction, Incubation, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Elevated Alpha 1(I) to Alpha 2(I) Collagen Ratio in Dermal Fibroblasts Possibly Contributes to Fibrosis in Systemic Sclerosis
doi: 10.3390/ijms23126811
Figure Lengend Snippet: COL1A1 overexpression affects COL1A2 transcript levels in fibroblasts. ( A ) Relative mRNA levels of type 1 procollagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector were determined using real-time PCR. The mean value of the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control ( B ) Protein levels of type I collagen in control fibroblasts treated with virus-containing medium with a control or COL1A1 expression vector are shown. The graph shows the mean relative type I collagen levels. The mean value in the control vector was set as 1 (n = 3 per group). * p < 0.05 versus the control. ( C ) Control fibroblasts transfected with virus-containing medium with a control or COL1A1 expression vector were incubated for 12 h after treatment with 2.5 μg/mL actinomycin D. COL1A2 mRNA expression was analyzed using real-time PCR (and normalized to GAPDH). The values in untreated fibroblasts were set as 100% (n = 3 per group). * p < 0.05.
Article Snippet:
Techniques: Over Expression, Expressing, Plasmid Preparation, Real-time Polymerase Chain Reaction, Transfection, Incubation